Figure 6.

LPS/MSU challenge of animals results in decreased cytokine levels in comparison to LPS stimulation. Activation of the inflammasome was triggered in wild-type (wt) (A, B) or Trif-/- knockout (KO) (C, D) mice by the intraperitoneal injection of MSU at 1 mg/animal. Optionally, the NLRP3 inhibitor MCC950 was given intraperitoneally 1 h prior to MSU. Two hours after MSU injection, animals were administered 0.75 mg/kg LPS intraperitoneally. Four hours later, blood was collected for cytokine detection using ELISA (A, C), while peritoneal lavage obtained cells were used for MyD88 detection (B, D). Unpaired two-tailed t-test with Welch’s correction was used for comparison of TRIF-KO populations treated with LPS and MSU/LPS and unpaired two-tailed t-test was used otherwise. **p < 0.01, ***p < 0.005.