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. 2022 Jan 5;12:790258. doi: 10.3389/fimmu.2021.790258

Figure 7.

Figure 7

Constitutively present MyD88 cleavage fragment in the Waldenström’s macroglobulinemia (WM) cell line and lack of signaling inhibition by TIRL265P. MWCL-1 cells or B-lymphocytes were lysed and blotted for MyD88 (A) or caspase-1 (D) detection. MWCL-1 cells were seeded into fresh media, and inhibitors were added to the cells for 24 h. Supernatants were collected and the amounts of cytokines determined using ELISA. Data are represented as mean ± SD of at least 3 replicates (B, C). HEK293 cells were transfected with MyD88 cleavage fragments (hMyD88Δ1-148 or hMyD88Δ1-148L265P; 1, 5, 10, 25 ng/well) and reporter plasmids. Cells were lysed after 16 h and NF-κB activation was measured using luciferase assay. Data are represented as mean ± SD of at least 3 replicates (E). Experiments were repeated at least three times with similar results. One-tailed unpaired t-test was used for statistical analysis, ***p < 0.005.