TABLE 1.

Oligonucleotide primers for Campylobacter MLST

Locus	Dideoxyoligonucleotide primer			Amplicon size (bp)
	Function	Name and sequence
		Forward	Reverse
asp	Amplification	asp-A9, 5′-AGT ACT AAT GAT GCT TAT CC-3′	asp-A10, 5′-ATT TCA TCA ATT TGT TCT TTG C-3′	899
	Sequencing	asp-S3, 5′-CCA ACT GCA AGA TGC TGT ACC-3′	asp-S6, 5′-TTA ATT TGC GGT AAT ACC ATC-3′
gln	Amplification	gln-A1, 5′-TAG GAA CTT GGC ATC ATA TTA CC-3′	gln-A2, 5′-TTG GAC GAG CTT CTA CTG GC-3′	1,262
	Sequencing	gln-S3, 5′-CAT GCA ATC AAT GAA GAA AC-3′	gln-S6, 5′-TTC CAT AAG CTC ATA TGA AC-3′
glt	Amplification	glt-A1, 5′-GGG CTT GAC TTC TAC AGC TAC TTG-3′	glt-A2, 5′-CCA AAT AAA GTT GTC TTG GAC GG-3′	1,012
	Sequencing	glt-S1, 5′-GTG GCT ATC CTA TAG AGT GGC-3′	glt-S6, 5′-CCA AAG CGC ACC AAT ACC TG-3′
gly	Amplification	gly-A1, 5′-GAG TTA GAG CGT CAA TGT GAA GG-3′	gly-A2, 5′-AAA CCT CTG GCA GTA AGG GC-3′	816
	Sequencing	gly-S3, 5′-AGC TAA TCA AGG TGT TTA TGC GG-3′	gly-S4, 5′-AGG TGA TTA TCC GTT CCA TCG C-3′
pgm	Amplification	pgm-A7, 5′-TAC TAA TAA TAT CTT AGT AGG-3′	pgm-A8, 5′-CAC AAC ATT TTT CAT TTC TTT TTC-3′	1,150
	Sequencing	pgm-S5, 5-GGT TTT AGA TGT GGC TCA TG-3′	pgm-S2, 3′-TCC AGA ATA GCG AAA TAA GG-3′
tkt	Amplification	tkt-A3, 5′-GCA AAC TCA GGA CAC CCA GG-3′	tkt-A6, 5′-AAA GCA TTG TTA ATG GCT GC-3′	1,102
	Sequencing	tkt-S5, 5′-GCT TAG CAG ATA TTT TAA GTG-3′	tkt-S4, 5′-ACT TCT TCA CCC AAA GGT GCG-3′
unc	Amplification	unc-A7, 5′-ATG GAC TTA AGA ATA TTA TGG C-3′	unc-A2, 5′-GCT AAG CGG AGA ATA AGG TGG-3′	1,120
	Sequencing	unc-S5, 5′-TGT TGC AAT TGG TCA AAA GC-3′	unc-S4, 5′-TGC CTC ATC TAA ATC ACT AGC-3′