Fig. 2.

Effect of U6atac knockdown on minor intron retention in NRVMs. (A) Relative expression of the minor splicing component U6atac 48 h after gapmeR-mediated knockdown in NRVMs. (B) Schematic illustration of the primer design for qPCR analysis. (C–F) Relative expression of minor introns (C), minor intron-flanking exons (D), major introns (E) and major intron-flanking exons (F) as detected by qPCR using primers depicted in B. Relative expression was calculated using as reference the geometric mean of Gapdh, Hprt1 and Eef1e1. Data are presented as mean±s.d. for 3 wells per condition. **P<0.01; ***P<0.001 (unpaired two-tailed Student's t-test).