Fig. 4.
Minor intron retention of Scn5a reduces translation into Nav1.5 and INa density. (A) Gene structure of Scn5a with its minor intron indicated in red. (B) Minor intron retention in Scn5a 48 h after U6atac knockdown measured by qRT-PCR. (C) Nav1.5 (Scn5a) detection by western blot and (D) its quantification. Data in B,D are presented as mean±s.d., 3 wells per condition. **P<0.01 (unpaired two-tailed Student's t-test). (E) Representative INa traces recorded from NRVMs transfected with control or anti-U6atac gapmeR. (F–H) Average INa–voltage relationships (F), INa–voltage dependence of activation (G) and inactivation (H). Insets show voltage protocols. Patch-clamp data are presented as mean±s.e.m.; n indicates number of cardiomyocytes. *P<0.05 (two-way repeated measures ANOVA followed by Holm–Sidak test for post hoc analyses).