Table 2.

List of sequences used for metabarcoding and target PCRs to screen blood, tissue and tick samples for bacteria

References: 1, Gofton et al. [17]; 2, Turner et al. [144]; 3, Lopez et al. [145]; 4, Caporaso et al. [146]; 5, Beard et al. [147]; 6, Anderson et al. [148]; 7, Paddock et al. [149]; 8, Kawahara et al. [82]; 9, Beati and Keirans 2001 [42].

Primer	Sequence (5′−3′)	Reference
Bacteria 16S rRNA
27F-Y	AGAGTTTGATCCTGGCTYAG	[1]
338R	TGCTGCCTCCCGTAGGAGT	[2]
338F	ACTCCTACGGGAGGCAGCAG	[3]
806R	GGACTACHVGGGTWTCTAAT	[4]
Bartonella 16S rRNA/ITS
438s	GGTTTTCCGGTTTATCCCGGAGGGC	[5]
1100as	GAACCGACGACCCCCTGCTTGCAAAGC	[5]
Anaplasmataceae 16S rRNA
EC9	TACCTTGTTACGACTT	[6]
EC12	TGATCCTGGCTCAGAACGAACG	[7]
A171a	GCGGCAAGCCTCCCACAT	[8]
IS58-1345r	CACCAGCTTCGAGTTAAACC	[8]
Ixodida 12S rRNA
T1B	AAACTAGGATTAGATACCCT	[9]
T2A	AATGAGAGCGACGGGCGATGT	[9]