Table 2.
Parameters for TraDIS data-sets in the current study.
|
Library (condition) |
Total reads1 |
Reads mapped (%)2 |
Total UIS |
Total seq len/Total UIS |
|---|---|---|---|---|
|
Input |
||||
|
UTI89_1 (input 1) |
11 574 067 |
10 372 421 (89.6) |
190 809 |
27.1 |
|
UTI89_2 (input 2) |
8 331 970 |
8 049 946 (96.6) |
149 998 |
27.8 |
|
UTI89_1+UTI89_2 (combined) |
19 906 037 |
18 422 370 (92.5) |
222 483 |
23.3 |
|
Output (urine) |
||||
|
UTI89_urineT20_1 |
7 552 213 |
7 267 934 (96.2) |
156 442 |
33.1 |
|
UTI89_urineT20_2 |
12 016 059 |
11 426 136 (95.1) |
170 470 |
30.4 |
|
UTI89_urineT40_1 |
9 835 979 |
9 456 427 (96.1) |
170 137 |
30.4 |
|
UTI89_urineT40_2 |
14 345 388 |
13 820 140 (96.3) |
107 583 |
48.1 |
|
Output (EZ-MOPS) |
||||
|
UTI89_MOPST20_1 |
8 113 704 |
6 891 908 (84.9) |
164 719 |
31.4 |
|
UTI89_MOPST20_2 |
6 051 946 |
5 323 289 (84.9) |
153 221 |
33.8 |
|
Output (LB) |
||||
|
UTI89_LBT20_1 |
10 073 550 |
9 319 007 (92.5) |
163 924 |
31.6 |
|
UTI89_LBT20_2 |
8 420 136 |
7 946 561 (94.4) |
159 500 |
32.5 |
|
Output (mice) |
||||
|
UTI89_V_1 (single mouse_V) |
7 157 630 |
6 873 996 (96.1) |
67 213 |
77.1 |
|
UTI89_V_2 |
12 049 930 |
11 465 874 (95.1) |
75 755 |
68.4 |
|
UTI89_V1+UTI89_V2 (V combined) |
19 207 560 |
18 339 871 (95.5) |
85 268 |
60.7 |
|
UTI89_W_1 (single mouse_W) |
6 535 767 |
6 059 389 (92.7) |
40 090 |
129.3 |
|
UTI89_W_2 |
7 192 774 |
6 846 954 (95.2) |
42 668 |
121.4 |
|
UTI89_W1+UTI89_W2 (W combined) |
13 728 541 |
12 906 342 (94.1) |
50 876 |
101.8 |
|
UTI89_Z_1 (11 mice) |
7 197 671 |
6 779 735 (94.2) |
84 124 |
61.6 |
|
UTI89_Z_2 |
7 962 478 |
7 570 978 (95.1) |
84 334 |
61.4 |
|
UTI89_Z1+UTI89_Z2 (Z combined) |
15 160 149 |
14 350 712 (94.7) |
95 732 |
54.2 |
1Number of sequence reads with matching online barcode.
2Number of mapped sequence reads against E. coli UTI89 genome (% of the raw data).
UIS, Unique Insertion Sites.