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. Author manuscript; available in PMC: 2022 Jan 19.
Published in final edited form as: J Physiol. 2020 Jun 3;598(13):2669–2683. doi: 10.1113/JP279595

Figure 6. Lipodystrophic muscle develops neither IMAT nor a contractile deficit at day 21 post-glycerol treatment.

Figure 6.

A, representative images of PDGFRα immunostaining identifying fibro/adipogenic progeitors (FAPs). B, quantification of FAPs per unit area indicates an increase with glycerol (G) treatment compared with saline (S) in both wildtype (WT) and lipodystrophic (LD) genotypes. C, Oil Red O staining of decellularized male glycerol-treated EDL depicting absence of IMAT in lipodystrophic (LD) muscle compared with littermate wildtype (WT) controls. D, predicted lipid volume from Oil Red O extraction indicates a significant increase with glycerol treatment (S: saline, G: glycerol) in WT mice only. E, peak tetanic tension is significantly reduced with glycerol treatment in WT mice only. F, representative images of WT and LD glycerol-treated EDL sections stained with haematoxylin & eosin (H&E) to visualize tissue morphology, Picrosirius red to visualize collagen (red) and laminin to visualize fibre areas (red-rimmed areas). Note the appearance of intramuscular adipocytes (unstained areas) in WT only but centrally placed nuclei (blue in fibre centres) in both genotypes. G, quantification of Picrosirius red staining indicates increased collagen deposition with glycerol treatment in both genotypes. H, quantification of fibre cross-sectional area (CSA) on laminin-stained sections indicates decreased fibre CSA with glycerol treatment in both genotypes. N = 6; *P < 0.05, **P < 0.01 by Bonferroni post-test on two-way ANOVA.