Figure 2. Photomicrographs under UV light (with red filter) and phase contrast.

Chlamydia transformants generated in this study, grown in mouse McCoy fibroblasts. Scale bars represent 20µM. A. Serial passage of SWFP-/pSW2-RiboA-C9. The whole harvest was passaged at each stage, but the frequency of transformants did not expand. T1-T4 are representative passage numbers. At T1, most cells contain inclusions with typical aberrant body phenotypes consistent with non-transformed Chlamydia grown under penicillin selection. By T2 these non-transformed Chlamydia were no longer present consistent with their penicillin-sensitive phenotype and by passage 6, the transformants were also lost. B. SWFP- transformed with pSW2-RiboA-C9Q. Successful expansion in cell culture; representative image from passage 6 is shown.