Table 1. Bacteria and plasmids used in this study.

	Name	Features	Reference/Source
E. coli	DH5α	Basic E. coli host for cloning and maintenance of plasmid vectors.	Hanahan et al., 1985 26 Grant et al., 1990 27 . Supplied by ThermoFisher.
	C2925	Methyltransferase deficient (dam-/dcm-) chemically competent E. coli	Supplied by New England Biolabs (C2925I)
C. trachomatis	SWFP-	Plasmid-free urogenital strain, serovar F.	Wang et al., 2013 24 . Supplied by the Chlamydia Biobank (CT107)
	L2(25667R)	Plasmid-free LGV strain L2	Peterson et al., 1990 25 . Supplied by the Chlamydia Biobank (CT109)
Plasmids	p2TK2-SW2-mCh	Chlamydia shuttle vector carrying Spectinomycin resistance marker aadA2 and mCherry (red fluorescent protein).	Cortina et al., 2019 28 . Provided by Prof Isabelle Derre (University of Virginia, USA)
	pSW2-RiboA-C9	As for p2TK2-SW2-mCh but also carrying the Himar1 transposon and C9 hyperactive transposase under control of the tet promoter and riboswitch A	This study
	pSW2-RiboA-C9Q	As for pSW2-RiboA-C9 but with amino acid substitution R131Q	This study