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Determination of the plasma elimination half-life of TIRO-CyAl5.5. After injecting TIRO-CyAl5.5 via the tail vein in normal C57BL/6J mice, serial blood sampling was performed via the carotid artery and (A) CyAl5.5 levels were measured by NIRF imaging. (B) Blood half-life was 5.65 min (95% CI, 4.54–7.47).
