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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Curr Protoc. 2021 Feb;1(2):e16. doi: 10.1002/cpz1.16

Figure 1: Auxin-inducible degradation.

Figure 1:

In tissues that express Arabidopsis TIR1 ubiquitin ligase transgene (red) via a tissue specific promoter, addition of auxin (blue) acts like glue to mediate the interaction between TIR1 and the degron-tagged endogenous protein (green). As a result, TIR1 engages machinery in the ubiquitin ligation pathway (grey) and the degron-tagged protein is modified by ubiquitin (yellow). This ubiquitination process targets the degron-tagged protein to the proteasome for degradation and protein function is eliminated. Tissues that do not express TIR1 continue to express the degron-tagged protein at wild-type levels. Eliminating auxin from the system can restore degron-tagged protein expression levels in tissues expressing TIR1 to wild-type levels over time. Therefore, this method provides a rapid and reversible method to eliminate protein expression in specific tissues.