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. Author manuscript; available in PMC: 2022 Jan 19.
Published in final edited form as: Cell Rep. 2022 Jan 4;38(1):110199. doi: 10.1016/j.celrep.2021.110199

Figure 2. SHIV RNA detection by in situ confocal staining.

Figure 2.

(A) Representative confocal image of whole lymph node showing SHIV RNA (green), CD20 (orange), and cellular nucleic acids (blue). Scale bar: 500 μm (B) Magnified area from (A) that shows an extra-follicular cell infected with SHIV (top) and a further magnified image (bottom) that shows the virally infected cell associated with T cell markers of CD3 (cyan) and CD4 (red). (C) Magnified area from (A) that shows an intra-follicular cell that is infected with SHIV (top) and a further magnified image (bottom) that shows the virally infected cells associated with T cell markers of CD3 (cyan) and CD4 (red). (D) Magnified area from (A) that shows the intra-follicular area filled with free virus and a further magnified image (bottom) shows the free virus associated with the FDC network (pink). Scale bars: 20 μm (top) and 10 μm (bottom). Additional detailed, merged images are shown in figure S4. (E) RNAscope quantification of SHIV gag mRNA containing cells and free virions in lymph nodes obtained from SHIV-infected animals pre- and post-infusion of the trispecific antibody. Indicated p values were calculated using a nonparametric Kruskal-Wallis test.