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. Author manuscript; available in PMC: 2022 Jan 19.
Published in final edited form as: Cell Rep. 2022 Jan 4;38(1):110199. doi: 10.1016/j.celrep.2021.110199

Figure 4. Effect of monoclonal and trispecific antibodies on replication of HIV-1 from infected donor CD4+ T cells.

Figure 4.

Activated CD4+ T cells from five viremic HIV-1 infected donors were cultured in the presence of the indicated antibodies for 2-3 weeks and viral replication was quantified by measuring HIV-1 p24 protein and gag RNA levels in the culture supernatants. (A) HIV-1 p24 protein levels (% of control, where control is denoted as 100% for each time point) in the culture supernatants were quantified by measuring HIV-1 p24 protein levels in the culture supernatant over time by ELISA. The levels shown are means of the levels measured in triplicate wells for each antibody from one experiment performed for each donor. (B) HIV-1 gag RNA levels in the culture supernatants were quantified at the end of the incubation using real time qPCR. Viral load (copies/ml) for each donor were as follows: donor 1: 4740, donor 2: 4503, donor 3: 3488, donor 4: 60,910, donor 5: 1810.