Fig. 4.

Embryonic axes of Acer pseudoplatanus L. (MC of 50%) were subjected to accelerating ageing conditions (storage at 37 and 45 °C; t0) and post-ageing recovery for 18 h at 4 °C (t18). A Survival (n = 5) and B regrowth (n = 5) were evaluated in in vitro cultures, C DNA strand breaks were evaluated using the alkaline comet assay (n = 3). Nuclei were isolated in Sörensen buffer supplemented with 0.5 mM Na₂EDTA and 0.5% DMSO. Values labelled with different letters are significantly different at p ≤ 0.05, according to Duncan test. Data represent mean ± SE