Fig. 7.

Pseudo-virus neutralization in Vero E6 cells following heterologous prime-booster SARS-CoV-2 vaccination. Sprague Dawley rats (8-weeks-old, 10 per treatment group) were injected subcutaneously once or twice, at a 14-day interval, with adjuvanted S1 subunit. Two weeks after the last priming dose, oral MigVax-101 was administered once or twice, at 14-day interval. Control rats received one or two injections of S1, followed by one or two oral doses of PBS or a third injected dose of S1. Presented are results from two-fold serial dilutions of heat inactivated serum collected 14 days after the third immunization were incubated with pseudo-SARS-CoV-2 virus. The y-axis corresponds to the titter in rat sera of antibodies neutralizing virus infection of Vero E6 cells. The neutralization assay was performed in triplicates for each rat and the presented values show the mean ± standard deviation. Statistical tests performed to determine p values are indicated in the figure.