REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Streptavidin-PE	ThermoFisher Scientific	Cat#S21388
Streptavidin-APC	BD Biosciences	Cat#554067; RRID:AB_10050396
Fixable Viability Stain 700	BD Biosciences	Cat#564997; RRID:AB_2869637
Human Fc block	BD Biosciences	Cat#564220; RRID:AB_2869554
Stain brilliant buffer	BD Biosciences	Cat#566349; RRID:AB_2869750
B-ly4 (BV421) [Anti-CD21]	BD Biosciences	Cat#562966; RRID:AB_2737921
FA6-2 (BV510) [Anti-IgD]	BD Biosciences	Cat#563034; RRID:AB_2737966
HI10A (BV605) [Anti-CD10]	BD Biosciences	Cat#562978; RRID:AB_2737929
SJ25C1 (BV711) [Anti-CD19]	BD Biosciences	Cat#563036; RRID:AB_2737968
2H7 (APC-H7) [Anti-CD20]	BD Biosciences	Cat#560734; RRID:AB_1727449
G18-145 (BV786) [Anti-IgG]	BD Biosciences	Cat#564230; RRID:AB_2738684
N-T271 (PE-CF594) [Anti-CD27]	BD Biosciences	Cat#562297; RRID:AB_11154596
HIT2 (PE-Cy7) [Anti-CD38]	BD Biosciences	Cat#560677; RRID:AB_1727473
G46-6 (BB515) [Anti-HLA-DR]	BD Biosciences	Cat#564516; RRID:AB_2732846
SK7 (BB700) [Anti-CD3]	BD Biosciences	Cat#566575; RRID:AB_2860004
AffiniPure Goat Anti-Human IgG, F(ab')2 Fragment Specific	Jackson ImmunoResearch	Cat#109-005-097; RRID:AB_2337540
2A3 (APC) [Anti-CD25]	BD Biosciences	Cat#340939; RRID:AB_400551
L106 (PE) [Anti-CD134]	BD Biosciences	Cat#340420; RRID:AB_400027
RPA-T4 (Alexa 700) [Anti-CD4]	Biolegend BD Biosciences	Cat#557922; RRID:AB_396943
RPA-T8 (BV650) [Anti-CD8a]	Biolegend	Cat#301041; RRID:AB_11125174
4B4-1 (BV421) [Anti-CD137 or 4-1BB]	Biolegend	Cat#309819; RRID:AB_10895902
FN50 (PE-Cy7) [Anti-CD69]	Biolegend	Cat#310911; RRID:AB_314846
A1 (FITC) [Anti-CD39]	Biolegend	Cat#328205; RRID:AB_940423
G034 × 103 (PerCP Cy5.5) [Anti-CD196/CCR6]	Biolegend	Cat#353406; RRID:AB_10918437
G025H7 (Alexa Fluor 647) [Anti-CXCR3]	Biolegend	Cat#353712; RRID:AB_10962948
MU5UBEE (SB436) [Anti-CD185/CXCR5]	Thermo Fisher	Cat#62-9185-4 2; RRID: AB_2724064
UCHT1 (AF532) [Anti-CD3]	Thermo Fisher	Cat#58-0038-4 2; RRID: AB_11218675
10.1 (FITC) [Anti-CD64/Fcγ receptor RI]	Invitrogen	Cat# 11-0649-42; RRID:AB_10544395
B73.1 (PE) [Anti-CD16/FcγRIII]	BD Biosciences	Cat#561313; RRID: AB_10643606
MφP9 (PerCP) [Anti-CD14]	BD Biosciences	Cat#340585; RRID:AB_400065
FLI8.26 (ACP) [Anti-CD32/FcγRII]	BD Biosciences	Cat#559769; RRID: AB_398665
Bacterial and virus strains
Staphylococcal Enterotoxin B from Staphylococcus aureus	Sigma-Aldrich Pty Ltd	S4881-1MG
SARS-Cov-2 Virus variant B.1.319 (D614G)	This paper	GISAID-ID: hCoV-19/Australia/NSW4713/2021
SARS-Cov-2 Virus variant B.1.17 (Alpha)	This paper	GISAID-ID: hCoV-19/Australia/NSW-R0066/2021
SARS-Cov-2 Virus variant B.1.351 (Beta)	This paper	GISAID-ID: hCoV-19/Australia/NSW4463/2021
SARS-Cov-2 Virus variant B.1.617.2 (Delta)	This paper	GISAID-ID: hCoV-19/Australia/NSW4605/2021
Biological samples
Convalescent donor blood samples	The Kirby Institute, UNSW	https://kirby.unsw.edu.au/project/natural-history-cohort-following-sars-cov-2-infection
Donor blood samples	Australian Red Cross Lifeblood	https://www.donateblood.com.au/
Chemicals, peptides, and recombinant proteins
TMB Chromogen Solution	ThermoFisher Scientific	Cat#002023
Imidazole	Sigma-Aldrich	Cat#I202
ExpiFectinamine	ThermoFisher Scientific	Cat#A14524
OptiMEM-1	ThermoFisher Scientific	Cat#31985070
Phosphate buffer saline	Gibco	Cat#10010023
HEPES buffer	Gibco	Cat#15630080
Sodium azide	Sigma	Cat# S2002
Critical commercial assays
Biotin Protein Ligase	Genecopeia	Cat#BI001
Biotinylation Kit NHS-Linker	ThermoFisher Scientific	Cat#20217
Experimental models: Cell lines
Expi293-Freestyle cells	ThermoFisher Scientific	Cat#A14527
ACE2-TMPRSS2-Hek293T cells	A/Prof Stuart Turville	N/A
VeroE6	CellBank Australia	CODE: 85,020,020206
THP-1 cells	ATCC	Cat#202 TIB
Recombinant DNA
pCAGGS-SARS-CoV-2-RBD	Dr Markus Hoffmann	N/A
pCAGGS-SARS-CoV-2-Spike	Dr Markus Hoffmann	N/A
Software and algorithms
GraphPad Prism	GraphPad	N/A
FlowJo version 10.7.1	Tree Star, Inc	N/A
SPSS 25	IBM	N/A
R 4.0.2	The R Foundation	N/A
Incarta Image analysis software	Cytiva	N/A
Other
Streptavidin coated Alexa-488 microbeads	Spherotech	Cat# VFP-0552-5