TABLE 2.
Primers designed for multiplex PCR from specific genomic regions (GRs) in the study to evaluate the prevalence of dominant Elizabethkingia anophelis strains
| Region | Primer name | Sequence (5′–3′) | Size (mer.) | Tm (°C) | GC (%) | Product size (bp) | Target gene |
|---|---|---|---|---|---|---|---|
| GR3 | Ez_R3-1F | gAggCAAATTggAAAgAgT | 19 | 46.8 | 42 | 1026 | From the CMP/dCMP deaminase gene to the zinc binding domain-containing protein gene |
| GR3 | Ez_R3-1R | TTCTgggTAAgTTggTgT | 18 | 45.8 | 44 | ||
| GR4 | Ez_R4-1F | TATTgTgAgCCCTTCgTT | 18 | 45.8 | 44 | 681 | Type II, N-methyl DNA methyltransferase gene |
| GR4 | Ez_R4-1R | CATTTCCgTCTTggTCTT | 18 | 45.8 | 44 | ||
| GR5 | Ez_R5-1F | CgggACATAACgCAAATA | 18 | 45.8 | 44 | 249 | N-acetylmuramonyl-l-alanine amidase gene |
| GR5 | Ez_R5-1R | gCCAgTTTCTAACATCgAA | 19 | 46.8 | 42 |