TABLE 2.

The details of MGEs captured by the pBACpAK entrapment vector

Strain (accession no.)	MGE/mutation	Location on pBACpAK (position)	Size (bp)	% similarity (ISFinder/NCBI)			Direct repeat
				BLASTN	BLASTX	Accession no.
E. coli MDS-K46-62-TC strains
Tet-11	Tn7350	cI repressor (2874)	5,812	99.12		HF955507
	Recombinase family protein		570		99	WP_195767670
	Hypothetical protein		849		91	SAP93263
	RepB family plasmid replication initiator protein		720		99	WP_087871684
	Transcriptional regulator ardK		342		100	ACI63129
	Zinc metalloproteinase Mpr		465		99	CDF32051
	ISSbo1		1,709	96		CP001062
Tet-21	Tn7351	cI repressor (2963)	4,796	99.25		HF955507	GAAC
	Hypothetical protein		849		95	SXT51837
	RepB family plasmid replication initiator protein		720		99	WP_087871684
	Transcriptional regulator ardK		342		100	ACI63129
	Zinc metalloproteinase Mpr		465		99	CDF32051
	ISSbo1		1,709		94	CP001062
E. coli MDS-50675619-TC-Tet-4	ISSbo1	cI repressor (2976)	1,709	96	98	CP001062	GAAC
E. coli MDS-50825040-TC strains
Tet-2-1	ISKpn14	cI repressor (3127)	768	100	100	CP000649	CGGCGTTA
Tet-2-3 (SAMN21542910)	ISKpn25	Between tet(A) and oriV (4976)	8,154	100	100	NC_009650	TATTTATC
	Deletion	cI repressor (3174–3179)	6
Tet-3-1 (SAMN21542911)	K. pneumoniae blaNDM-1 plasmid p2	cI repressor (3126)	118,959	99.99		CP009115	TAACGCCG
Tet-3-7 (SAMN21542912)	ISKpn25_1	cI repressor (3224)	8,154	100	100	NC_009650	CATTTTTC
	ISKpn25_2	Between tet(A) and oriV (4980)	8,154	100	100	NC_009650	TTTTATTT
Tet-4-13 (SAMN21542913)	ISKpn25	cI repressor (3294)	8,154	100	100	NC_009650	CTTTTTTG
Tet-4-38 (SAMN21542914)	ISKpn25_1	cI repressor (3230)	8,154	100	100	NC_009650	AAAAAAAG
	ISKpn25_2	Between tet(A) and oriV (4941)	8,154	100	100	NC_009650	AATCTTCT
Tet-4-52 (SAMN21542915)	ISKpn25	cI repressor (3227)	8,154	100	100	NC_009650	AAAGAAAA
E. coli MDS-50627996-TC-Tet-2 (SAMN21542916)	IS26	sopA (8066)	820	100	100	X00011	CAGATCTT
	Deletion	cI repressor (2305–3090)	786