Figure 4.

Identification of centromere tandem repeat sequences based on bioinformatic screening and FISH analyses. Schematic representation of the physical locations of Mn-175 nt candidate centromere sequences on the chromosomes of M. notabilis (a) and Ma-175 nt candidate centromere sequences on the chromosomes of M. alba ‘Heyebai’ (b). FISH mapping of Mn-175 nt on the diakinesis chromosomes of M. notabilis (c) and ‘Lunjiao109’ (d), in meiotic metaphase I of ‘Lunjiao109’ (e), and in meiotic metaphase II of ‘Lunjiao109’ (f). Arrows in (f) indicate that Mn-175 nt FISH signals were clearly located in the centromere regions of the cruciform bivalents. Chromosomes were stained with DAPI (blue). Scale bars represent 5 μm.