(A) Volcano plot showing the significance versus the log2 fold change in SUM159 tumor masses, by comparing FMD versus AL. Up- and downregulated genes (|log2FC| > 0.58 and adj. p < 0.05) are displayed in red and green, respectively. Deregulated genes involved in PI3K-AKT-mTOR and CycD-CDK4/6 pathways are highlighted. Significantly deregulated genes are reported in bold.
(B) Growth of SUM159 xenografts in 8-week-old female NOD scid (NSG) mice treated with AL diet or FMD, alone or combined with pictilisib (100 mg/kg, 5 consecutive days a week by oral gavage), ipatasertib (75 mg/kg, 5 consecutive days a week by oral gavage), or rapamycin (2 mg/kg, every other day i.p.), as single or double treatments (n = 8–12). Survival curves are reported.
p values were determined by log-rank (Mantel-Cox) test (B). See also Figure S6.