KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
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| Antibodies | ||
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| FITC-conjugated anti-murine CD24 antibody | Miltenyi | Cat. #: 130–102-731; RRID: AB 2656573 |
| PE-conjugated anti-murine CD44 antibody | Miltenyi | Cat. #: 130–102-606; RRID: AB 2658181 |
| FITC-conjugated anti-human CD24 antibody | Miltenyi | Cat. #: 130–112-844; RRID: AB2656554 |
| Vioblue-conjugated anti-human CD44 antibody | Miltenyi | Cat. #: 130–113-899; RRID: AB 2726390 |
| Cleaved caspase-3 antibody (Asp175) | Euroclone | Cat. #: BK9661S |
| Mouse Anti-Vinculin Monoclonal Antibody 1:10000 |
Sigma-Aldrich | Cat. #: V9131; RRID: AB 477629 |
| Rabbit anti-GLUT1 monoclonal antibody 1:10000 |
Cell Signaling | Cat. #: 12939; RRID: AB 2687899 |
| Rabbit anti-CREB monoclonal antibody 1:1000 | Cell Signaling | Cat. #: 4820S; RRID: AB 1903940 |
| Rabbit anti-phosphoCREB monoclonal antibody 1:1000 | Cell Signaling | Cat. #: 9198S; RRID: AB 2561044 |
| Rabbit anti-KLF5 polyclonal antibody 1:1000 | Abcam | Cat. #: AB137676; RRID: AB 2744553 |
| Rabbit anti-G9A/EHMT2 monoclonal antibody 1:1000 | Euroclone | Cat. #: BK68851T |
| Mouse anti-histone H3dimethylK9 monoclonal antibody 1:1000 | Abcam | Cat. #: AB1220; RRID: AB 449854 |
| Goat Anti-Mouse IgG 1:3000 | Biorad | Cat. #: 170–6516 |
| Goat Anti-Rabbit IgG 1:3000 | Biorad | Cat. #: 170–6515 |
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| Chemicals, peptides, and recombinant proteins | ||
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| Glucose transporter inhibitor IV, WZB117 | MERK | Cat. #: 400036 |
| Metformin | Sigma-Aldrich | Cat. #: D150959 |
| 2-Deoxy-D-Glucose | Sigma-Aldrich | Cat. #: D-6134 |
| 8-Bromoadenosine 3′,5′-cyclic monophosphates (8-Br-cAMP) | Cayman | Cat. #: 14431 |
| Alpelisib | MedchemTronica | Cat. #: HY-15244 |
| Rapamycin | MedchemTronica | Cat. #: HY-10219 |
| Pictilisb | MedchemTronica | Cat. #: HY-50094 |
| Ipatasertib | MedchemTronica | Cat. #: HY-15186 |
| Erythrosin B | Sigma-Aldrich | Cat. #: 200964 |
| Mammary epithelial basal medium (MEMB) | Lonza | Cat. #: CC-3151 |
| Methylcellulose | Sigma-Aldrich | Cat. #: M0512 |
| B-27 | GIBCO | Cat. #: 17504044 |
| Epidermal growth factor (EGF) | Biomol | Cat # BPS-90201–3 |
| Fibroblast growth factor (FGF) | Peprotech | Cat. #: 100–18B |
| Insulin growth factor 1 (IGF-1) | Peprotech | Cat. #: 100–11 |
| Hyaluronidase | Sigma-Aldrich | Cat. #: H4272 |
| Collagenase | Sigma-Aldrich | Cat. #: C2674 |
| Red blood cell lysing buffer hybrid-max | Sigma-Aldrich | Cat. #: R7757 |
| Protease inhibitor cocktail set III EDTA-free | Calbiochem | Cat. #: S39134 |
| PhosStop | Roche | Cat. #: 04906845001 |
| 1%penicillin/streptomycin | Biowest | Cat. #: L0022 |
| RPMI 1640 medium | Biowest | Cat #: L0500 |
| Ham’s F-12 medium | GIBCO | Cat. #: 11–765-054 |
| FBS | Biowest | Cat. #: S1810 |
| DMEM no glucose | Life Technologies | Cat. #: 11966025 |
| Glucose | Sigma-Aldrich | Cat. #: G8769 |
| Hydrocortisone | Sigma-Aldrich | Cat. #: H4001 |
| Insulin | Sigma-Aldrich | Cat. #: 11376497001 |
| L-glutamine | Biowest | Cat. #: X0550 |
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| Critical commercial assays | ||
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| ALDEFLUOR kit | STEMCELL technologies | Cat. #: 01700 |
| EasySep Mouse Epithelial Cell Enrichment Kit |
STEMCELL technologies | Cat. #:19868 |
| BCA assay | Thermo Fisher Scientific | Cat. #: 23225 |
| miRNeasy Mini Kit | QIAGEN | Cat. #:217004 |
| SuperScript Vilo cDNA synthesis kit | Invitrogen | Cat. #:11754050 |
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| Deposited data | ||
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| RNA-seq data | Gene Expression Omnibus (GEO) | GEO: GSE184452 |
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| Experimental models: Cell lines | ||
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| SUM159 PT | Asterand | N/A |
| 4T1 | ATCC | N/A |
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| Experimental models: Organisms/strains | ||
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| NOD scid gamma mice | Charles River | N/A |
| Balbc/Ola Hsd mice | Envigo | N/A |
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| Oligonucleotides | ||
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| Primer for NANOG (hs02387400_g1) | ThermoFisher | Cat. #: 4331182 |
| Primer for OCT4 (hs00742896_s1 | ThermoFisher | Cat. #: 4331182 |
| Primer for TBX3 (hs00195612_m1) | ThermoFisher | Cat. #: 4331182 |
| Primer for KLF2 (Hs00360439_g1) | ThermoFisher | Cat. #: 4331182 |
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| Software and algorithms | ||
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| Kaluza analysis software (Beckman coulter, version 2.0) | Kaluza Software | N/A |
| FlowJo (version 10.7.1) | FlowJo software | N/A |
| ImageJ (version 1.50i). | ImageJ software | N/A |
| Image Lab (version 5.2.1) | Image Lab software | N/A |
| ELDA | ELDA software | N/A |
| GraphPad Prism V9 | GraphPad software | N/A |
| R (version 4.0.2) | R software | N/A |
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| Other | ||
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| Standard diet (VRFI (P)) | Special Diets Services (SDS) | Cat #: 801900 |