TABLE 2.
Primers and plasmids used in this study
| Designation | Sequence or description | Source or reference |
|---|---|---|
| Primers | This study | |
| NTHi gor flanking F | CTAGAGATCTTTATTTTTTATATGCCAAGATTTCG | This study |
| NTHi gor kof XhoI | CTCGAGAATGCAGATGGTTCACTTACTGTAA | This study |
| NTHi gor flanking R | CTAGAGATCTACGCTAATTGGTTATAAGATTAATT | This study |
| NTHi gor kor XhoI | CTCGAGAATTTCAGATGGGGTAGAATTGGT | This study |
| NTHi bcp flanking F | CTAGAGATCTATGGTACAACTTTTTGATTT | This study |
| NTHi bcp kof XhoI | CTCGAGAGTTGCGGAGCAATTTGGCG | This study |
| NTHi bcp flanking R | CTAAAACGCGACTGTAAGATCTCTAG | This study |
| NTHi bcp kor XhoI | CTCGAGTCAGGATCAGAAAGCAATGTGAAAT | This study |
| NTHi trxB flanking F | TAAAATTCCAACAAGCATTA | This study |
| NTHi trxB kof XhoI | CTCGAGAATTGCAAGTACAGTGCATTTAATCCAC | This study |
| NTHi trxB flanking R | AAGCCTGCAGCCCAG | This study |
| NTHi trxB kor XhoI | CTCGAGAAGTAAAGGGCTTCTTCCACCG | This study |
| NTHi pdgX flanking F | TTATCATATTTAATAACTTCTTTGT | This study |
| NTHi pdgX xof XhoI | CTCGAGAATTCACCATTACCATCTGGAATGAA | This study |
| NTHi pdgX flanking R | AGTAACATTTTCTCGTTGAA | This study |
| NTHi pdgX xor XhoI | CTCGAGAAGGCATGGGTATGTTAGTTGGT | This study |
| NTHi tpx flanking F | ACGAACGTTTTCCTGAGCGTG | This study |
| NTHi tpx xof XhoI | CTCGAGGCCCTTTGCTCAAGCTCGTT | This study |
| NTHi tpx flanking R | TATACTCGCTCTACAACCGCT | This study |
| NTHi tpx xor XhoI | CTCGAGGCTGAAATACAAAGCACAACAGT | This study |
| SpecF XhoI | CTCGAGAGAATTCGGATCCCGGGATATCT | This study |
| SpecR XhoI | CTCGAGGAATTCCCGGGATCGGATCC | This study |
| CmF XhoI | CTCGAGATGGAGAAAAAAATCACTGGATATACC | This study |
| CmR XhoI | CTCGAGTTACGCCCCGCCCTG | This study |
| Plasmids | This study | |
| pCR2.1 | Cloning vector | Invitrogen |
| pCR2.1gor | gor clone | This study |
| pCR2.1bcp | bcp clone | This study |
| pCR2.1trxB | trxB clone | This study |
| pCR2.1pdgX | pdgX clone | This study |
| pCR2.1tpx | tpx clone | This study |
| pCMr | Plasmid containing chloramphenicol resistance cassette used to generate mutants | 60 |
| pSpec | Plasmid containing spectinomycin resistance cassette used to generate mutants | 61 |
| pCR2.1gor::spec | gor null mutant | This study |
| pCR2.1bcp::cm | bcp null mutant | This study |
| pCR2.1trxB::spec | trxB null mutant | This study |
| pCR2.1pdgX::cm | pdgX null mutant | This study |
| pCR2.1tpx::spec | tpx null mutant | This study |
| pACYC184 | Cloning vector used to complement mutants | 73 |
| pACYC184gor | gor complementation | This study |
| pACYC184bcp | bcp complementation | This study |
| pACYC184trxB | trxB complementation | This study |
| pACYC184pdgX | pdgX complementation | This study |
| pACYC184tpx | tpx complementation | This study |