Fig 1. Detection of leptospirosis using the RPA-CRISPR/Cas12a-FBDA.
(A) Schematic representation of the RPA-CRISPR/Cas12-FBDA’s workflow (Created with BioRender.com). (B, C) The (B) LOD and (C) cross-reactivity of the RPA-CRISPR/Cas12a targeting lipL32 gene against several infectious diseases with similar clinical manifestations as leptospirosis.