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. Author manuscript; available in PMC: 2022 Jan 19.
Published in final edited form as: Science. 2021 Jun 25;372(6549):eabg0269. doi: 10.1126/science.abg0269

Fig. 1. A genome wide CRISPR screen identifies multiple components of the FLCN-FNIP2-Rag-Ragulator as needed for caspase-8-mediated pyroptosis.

Fig. 1.

(A) Schematic workflow of the genome-wide CRISPR screening procedure in iBMDMs. (B) -lgRRA values calculated by the MAGeCK algorithm indicate the relative enrichment of genes after treatment with LPS electroporation (x axis) or LPS/5z7 (y axis) relative to controls in positive selection. The dotted lines represent p-value of 0.01. (C) List of sgRNA hits from the screening in iBMDMs treated with LPS/5z7. Corresponding genes targeted with multiple sgRNAs and fold enrichments are shown. Color code: genes encoding for TNF receptor, green; FLCN-FNIP2-Rag-Ragulator complex, red; mitochondrial electron transport complex I, blue. (D) Diagram of FLCN-FNIP2-Rag-Ragulator complex.