Skip to main content
. Author manuscript; available in PMC: 2022 Jan 19.
Published in final edited form as: Science. 2021 Jun 25;372(6549):eabg0269. doi: 10.1126/science.abg0269

Fig. 2. FLCN-FNIP2-Rag-Ragulator gene deficient cells are resistant to LPS/5z7 treatment, but sensitive to inflammasome-induced pyroptosis.

Fig. 2.

(A) The indicated iBMDMs were challenged with LPS or LPS/5z7. Cell death was measured by LDH release after 2.5 hr. Specific gene ablation was confirmed and Gsdmd cleavage was examined by immunoblot, shown in the bottom panel. (B-C, F-G) The indicated iBMDMs were treated with TNF, TNF/5z7 (B-C) or LPS/Nigericin (F-G). Cell death was measured by LDH release after 2.5 hr (B-C) or 1.5 hr (F-G). (D-E) The indicated iBMDMs were electroporated with LPS. Cell death was assessed and calculated by measuring ATP level after 2.5 hr. Gsdmd cleavage and caspase-1 processing (during canonical inflammasome activation) were examined by immunoblot. Graphs show mean ± SEM of triplicate wells. Data are representative of at least three independent experiments. Data were analyzed using a two-tailed Student’s t test. **P < 0.01.