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. Author manuscript; available in PMC: 2022 Jan 19.
Published in final edited form as: Science. 2021 Jun 25;372(6549):eabg0269. doi: 10.1126/science.abg0269

Fig. 4. mTORC1 activity is not required for LPS/5z7 or inflammasome-triggered pyroptosis.

Fig. 4.

(A-B) iBMDMs pre-incubated with Rapamycin or Torin 1 for 2 hr were treated with LPS or LPS/5z7. Cell death was measured by LDH release after 2.5 hr (A), or by entry of PI into cells (B). (C) iBMDMs pre-incubated with AICAR for 2 hr were treated with LPS or LPS/5z7. Cell death was measured by LDH release 2.5 hr later. (D) iBMDMs pre-incubated with Rapamycin or Torin 1 for 2 hr were electroporated with LPS. Cell viability was assessed by measuring ATP level after 2.5 hr. (E) iBMDMs pre-incubated with Rapamycin or Torin 1 for 2 hr were treated with LPS or LPS/Nigericin and cell death was measured by LDH release 1.5 hr later. Gsdmd cleavage was examined by immunoblot. Graphs show mean ± SEM of triplicate wells. Data are representative of at least three independent experiments. Data were analyzed using a two-tailed Student’s t test. **P < 0.01.