Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 19;8(3):eabj5908. doi: 10.1126/sciadv.abj5908

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

PMC Copyright notice

Fig. 5. — (A) Morphology of hepatocyte-differentiated organoids in different time points. (B) Gene expression level of albumin upon hepatocyte differentiation (n = 5 to 6). EM, expansion medium. (C) Immunofluorescence staining for mature hepatic marker albumin in hepatocyte-differentiated organoids. Scale bar, 40 μM. (D) The secretion of albumin into supernatant quantified by ELISA (n = 5). (E) Immunofluorescence staining of albumin and HEV ORF2 in hepatocyte-differentiated organoids (FLO1 and ALO1 lines were used for differentiation). Scale bar, 20 μM. (F) HEV replication–related luciferase activity in hepatocyte-differentiated organoids (ALO1, n = 4). (G) Illustrating secretion of albumin and HEV from polarized hepatocyte-like cells in a transwell system. (H) Release of albumin into apical and basolateral compartments from polarized cells cultured in EM with cholangiocyte phenotype or DM with hepatocyte phenotype (n = 3). (I) Polarized release of HEV from hepatocyte-like cells differentiated from ICOs harboring p6 HEV genome (ALO1, n = 3). (J) Immunofluorescence staining of HEV ORF2 in Huh7 cells at 48 hours after inoculation of apical released virus from hepatocyte-like cells. Scale bar, 40 μM. Data are presented as means ± SD, **P < 0.01.