Table 2. 5-choice serial reaction time task (5-CSRTT) training and challenge stages.

The parameters stimulus duration (SD) and inter-trial interval (ITI, waiting time before stimulus) are listed for each of the five training stages (S1–5) and the subsequent challenge protocols on which performance was tested for 1 day each (C1–5). For the training stages, performance criteria which had to be met by an animal on two consecutive days to move to the next stage are listed on the right. See Materials and methods for the definition of these performance parameters.

5-CSRTT training
	Task parameters			Criteria for stage transition (two consecutive days)
Stage	SD (s)		ITI (s)	# correct	% correct	% accuracy		%omissions
S1	20		2	≥30	≥40	-	-
S2	8		2	≥40	≥50	-	-
S3	8		5			≥80	≤50
S4	4		5			≥80	≤50
S5	2		5			≥80	≤50
Challenges
C1		2	9	Impulsivity challenge
C2		1	5	Attention challenge 1
C3		0.8	5	Attention challenge 2
C4		2	5	Distraction: 1 s white noise within 0.5–4.5 s of ITI
C5		2	7, 9, 11, 13	Variable ITI: pseudo-random, equal distribution