Table 1. Statistics and success rates of automated on-grid lamellae milling with SerialFIB for five different cell types.
Sample | # Target sites | # Fine-milled lamellae | # Successfully transferred to TEM | Lamella thickness (nm) |
---|---|---|---|---|
Sum159 | 22 | 19 | 17 | 70–410 |
HeLa | 22 | 18 | 18 | 100–450 |
E. huxleyi | 9 | 9 | 9 | 175–470 |
C. reinhardtii | 16 | 10 | n/a | 140–350 |
S. cerevisiae | 8 | 8 | 8 | 190–300 |
Total | 77 | 64 | ||
Success rate (%) | 83.1 |
TEM, transmission electron microscope.