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Experimentally determined LOD of MRSA/SA ELITe MGB PCR assay. The LOD of S. aureus (ldh1 target) (A and C) and the LOD of methicillin resistance (mec target) (B and D) were determined using serial 5-fold dilutions of the methicillin-resistant S. aureus strain ATCC 43300 spiked in synovial fluids and fluidified bone and joint biopsy specimens.
