TABLE 1.
Comparison of culture, 16S rRNA PCR, and MRSA/SA ELITe MGB techniques for the detection of S. aureus in osteoarticular samples
| Technique | Sample statusa (n) |
|||
|---|---|---|---|---|
| S. aureus positive | S. aureus negative | Se (%)b | Sp (%)b | |
| Culture | ||||
| S. aureus positive | 28 | 0 | 70 | 100 |
| S. aureus negative | 12 | 168 | ||
| 16S rRNA PCR | ||||
| S. aureus positive | 23 | 0 | 57.5 | 100c |
| S. aureus negative | 17 | 165 | ||
| MRSA/SA ELITe MGB | ||||
| S. aureus positive | 39 | 15 | 97.5 | 91.1 |
| S. aureus negative | 1 | 153 | ||
a
The sample status was considered positive for S. aureus if the bacterial culture or 16S rRNA PCR result was positive; otherwise, the sample status was considered negative for S. aureus. The femA PCR was not used to assess samples status.
b
Se, sensitivity; Sp, specificity.
c
Three samples were not tested due to insufficient quantity.