TABLE 2.

Patients and samples determined to be positive for S. aureus using the MRSA/SA ELITe MGB PCR assay and negative by culture and 16S rRNA PCR

Patient	Sample	CT of MRSA/SA ELITe MGB assaya	femA PCR resultb	Detection of S. aureus by culture or 16S rRNA PCRc			Statusd
				Prior SA+ sample (days from detection)	SA+ samples during the surgery	Ulterior SA+ sample (days to detection)	Clinical	Antibiotic
1	1	31.1	+	+ (16)	0/5	–	+	+
	2	33.4	+
2	1	36.0	+	–	3/6	–	+	–
3	1	34.1	–	+ (15)	0/7	–	+	+
4	1	33.1	+	+ (22)	0/4	–	+	+
5	1	37.5	IQ	+ (20)	0/7	–	+	+
	2	31.1	IQ
6	1	32.7	IQ	+ (126)	0/3	–	+	+
7	1	33.9	IQ	+ (42)	0/5	+ (60)	–	+
8	1	30.9	+	+ (15)	0/5	–	–	–
	2	35.5	–
9	1	30.8	+	+ (18)	0/3	+ (15)	+	+
	2	32.3	+
	3	32.7	IQ
10	1	33.9	+	+ (26)	0/2	–	+	+

^aCycle threshold (C_T) value for S. aureus detection were determined. C_T values of <37.8 are considered positive.

^bIQ, insufficient quantity.

^cSA⁺, S. aureus positive.

^dThe clinical status was considered positive if the patient was determined to be septic by the referent infectious disease specialists at the time of surgery. The antibiotic status was considered positive if the patient was receiving treatment against staphylococci up to 14 days prior or at the time of the surgery.