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. 2022 Jan 5;12(1):e4275. doi: 10.21769/BioProtoc.4275

Figure 1. Cartoon representation of the expression vector integrated with sauUSIR gene.

Figure 1.

The sauUSIR gene is 2859 bp and codes of a 953 amino acid single polypeptide protein. This gene is integrated between NdeI and BamHI sites in a pHis 17 vector thus providing a C-terminal 6× Histidine tag for ease of purification.