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. 2022 Jan 5;12(1):e4275. doi: 10.21769/BioProtoc.4275

Figure 2. Representative 10% SDS-PAGE after Ni-NTA chromatography.

Figure 2.

Load 10 µL of sample (5 µL of the protein fraction mixed with 5 µL of 2× Laemmli sample buffer) on a 10% SDS-PAGE gel. The fractions with the most protein are pooled and dialysed. The SDS-PAGE gel is stained with Coomassie R-250.