Table 2. RT Master Mix.

RT Master Mix	[Units]	[Stock]	[Final]2	1 Rx (µl)	48Rx Master Mix (µl)1
Nuclease-free H2O	-	-	-	0.15	8.5
SSIV buffer	X	5	1	1.28	72
MgCl2	mM	1000	6	0.04	2.2
DTT	mM	300	5	0.11	6
Betaine	M	5	1	1.28	72
Template Switch Oligo	µM	100	1	0.06	3.6
RNase Inhibitor	U/µl	40	1	0.16	9
SuperScript IV	U/µl	200	10	0.32	18
-	-	-	Total	3.4	191.3