Table 4. Final PCR Master Mix.
| Final PCR Master Mix | [Units] | [Stock] | [Final]5 | 1 Rx (µl) | 24Rx Master Mix (µl)1 |
|---|---|---|---|---|---|
| Nuclease-free H2O | - | - | - | 8 | 250 |
| Nextera PCR Master Mix | X | 3.3333 | 1 | 15 | 468.75 |
| P5-ISPCR Primer | µM | 10 | 0.2 | 1 | 31.25 |
| - | - | - | Totals | 24 | 750 |
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