Quantification of HS and HA elements at hCMEC and MB231 under control and after VEGF treatment. (a) Diameter of HS and HA at hCMEC and at MB231. (b) Length of HS at hCMEC, thickness of HA at hCMEC, and thickness of HS and HA at MB231. The vertical dotted line separates the length of individual HS elements at hCMEC and the thicknesses of HA at hCMEC, and HS and HA at MB231. (c) Coverage of HS and HA at hCMEC and at MB231. The quantification method for the diameter, length, thickness, and coverage of HS and HA elements is described in Appendix D and in Data analysis of Methods section. Plots are mean ± SD. * p < 0.05.