Figure 4.

MEF2D is a target gene of miR-451a. (a) The target genes were predicted by ENCORI, miRWalk, and TargetScan. (b) The miR-451a putative binding sites and corresponding mutant sites of MEF2D. (c) Luciferase reported assay. (d) The mRNA expression of MEF2D detected by qRT-PCR in miR-451a mimics-treated PANC-1 cells. (e) The protein expression of MEF2D in miR-451a mimics-treated PANC-1 cells. (f) The protein expression of MEF2D in PANC-1 cells exposed to acidic conditions. Error bars, mean ± SD from three independent experiments. ^∗∗P < 0.01; ^∗∗∗P < 0.001.