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. 2022 Jan 12;2022:3966386. doi: 10.1155/2022/3966386

Figure 5.

Figure 5

Knockdown of MEF2D enhances MMP loss and ROS generation and inhibits stemness of PANC-1 cells. PANC-1 cells were transfected with sh-NC and sh-MEF2D for 48 h. (a) The oxygen consumption rate. (b) JC-1 staining was used to detect MMP loss of PANC-1 cells. (c) DCFH staining was used to detect ROS generation of PANC-1 cells. (d) The cell viability detected by CCK-8 assay. (e) Sphere formation assay was performed. (f) The expression of CD24, CD44, and ESA. Error bars, mean ± SD from three independent experiments. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.