Figure 7.

Tumor acidic microenvironment modulates the proliferation, migration, and stem cell stemness of PC through the miR-451a/MEF2D axis. Under acidic conditions, PANC-1 cells were transfected with miR-451a mimics, pc-MEF2D, and the corresponding control group. (a) The cell viability detected by CCK-8 assay. (b) Cell motility detected by wound healing assay. (c) Cell motility and invasion ability assessed by transwell assay. (d) Sphere formation assay was performed. (e) The expression of CD24, CD44, and ESA. Error bars, mean ± SD from three independent experiments. ^∗∗P < 0.01, ^∗∗∗P < 0.001, ^#P < 0.05, ^##P < 0.01, ^$P < 0.05, and ^$$P < 0.01.