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. 2022 Jan 20;3(1):101156. doi: 10.1016/j.xpro.2022.101156
REAGENT or RESOURCE SOURCE IDENTIFIER
Biological samples
COVID-19 PBMC samples (human, 24–81 years, 20 females, 23 males) Massachusetts General Hospital and Brigham’s and Women’s Hospital https://www.massgeneral.orghttps://www.brighamandwomens.org
COVID-19 and non-COVID-19 FFPE samples (human, 58–91 years, 4 females, 3 males) Beth Israel Deaconess Medical Center https://www.bidmc.org
Healthy control PBMC samples (human, 26–55 years, 4 females, 6 males) Blood Donor Center at Boston Children’s Hospital https://www.childrenshospital.org/ways-to-help/donate-blood
Chemicals, peptides, and recombinant proteins
K2 EDTA (K2E) Plus Blood Collection Tubes BD Vacutainer Cat. # 366643
Ficoll-Paque Plus GE Healthcare Life Sciences Cat. # GE17-1440-02
Dimethyl Sulfoxide (DMSO) Fisher Scientific Cat. # BP231-100
Trypan Blue Stain 0.4% Thermo Fisher Scientific Cat. # T10282
10% Buffered Formalin Phosphate Fisher Scientific Cat. # SF100-4
Xylene StatLab Cat. # 8400-1
Paraplast Plus - Tissue Infiltration/Embedding Medium McCormick Scientific Cat. # 39502004
β-mercaptoethanol Sigma-Aldrich Cat. # M3148
Isopropanol Molecular Biology Grade Fisher Scientific Cat. # BP26181
Critical commercial assays
RNeasy Mini kit (50) QIAGEN Cat. # 74104
AllPrep DNA/RNA FFPE kit QIAGEN Cat. # 80234
nCounter CAR-T Characterization Panel NanoString Cat. # XT-CSO-CART1-12
Coronavirus Panel Plus NanoString n/a
Deposited data
NanoString raw data Wang et al. (2021); Mendeley Data https://doi.org/10.17632/mkmv3mgz86.1
Software and algorithms
nSolver Analysis Software, 4.0 NanoString Technologies https://www.nanostring.com/products/analysis-solutions/ncounter-advanced-analysis-software/
R software, 4.0.2 R https://cran.r-project.org
GraphPad Prism, 9.3.0 GraphPad Software https://www.graphpad.com
Other
Thermolyne 16500 Dri Bath Marshall Scientific Cat. # TH-16500
SureSeal S MicroTubes MTC Bio Cat. # C2000
NanoDrop 1000 Spectrophotometer, 3.7.0 Thermo Fisher Scientific n/a
2100 Bioanalyzer Agilent Technologies Cat. # G2939BA
C1000 Touch Thermal Cycler with dual 48/48 Fast Reaction Module Bio-Rad Laboratories Cat. # 1851148
nCounter MAX Analysis System NanoString Technologies https://www.nanostring.com/products/ncounter-analysis-system/max-analysis-system/
Disposable Lab Coat Fisher Scientific Cat. # 12-893-0063A
Fisherbrand Disposable Face Mask Fisher Scientific Cat. # 12-888-001A
Bollé Safety Override OTG Goggles Fisher Scientific Cat. # 18-072-109
Nitrile Powder Free Examination Gloves Nest Cat. # 902021