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. 2001 Jan;39(1):191–195. doi: 10.1128/JCM.39.1.191-195.2001

FIG. 4.

FIG. 4

iRep1 PCR-based molecular typing of human and rat Leptospira strains isolated from an urban epidemic in Salvador, Brazil. DNA from boiled Leptospira culture pellets was amplified with the iRep1 primer. The following samples were identified as L. interrogans serovar copenhageni except for L1-133, shown in lane 3 (L. interrogans serovar canicola). The following clinical isolates of L. interrogans serovar copenhageni were evaluated: L1-130 (lane 5), L1-212 (lane 6), L8-38 (lane 7), L8-118 (lane 8), and L8-163 (lane 9). Rat isolates included R1-15 (lane 10), R1-98 (lane 11), and R1-147 (lane 12), and R1-152 (lane 13). Lanes 2 and 4 represent reference strains L. interrogans serovar canicola strain Hond Utrecht IV and L. interrogans serovar copenhageni strain Winjberg, respectively. The positions of the 100-bp size markers are represented in lanes 1 and 14.