Fig. 2. Temporal profiles of pregnancy pathways for gene sets from the gestational age model and independently identified gene sets for placenta, developing fetal heart and collagen extracellular matrix known to be involved in uterus and cervix growth over gestation.

a–d, Maternal plasma transcriptome fractions for gene sets averaged across all samples in each collection window. Gestational age model (a), placenta (b), developing heart (c) and collagen extracellular matrix (ECM) (d). Error bars correspond to the 95% confidence interval around the mean. CPM, counts per million. n = 93 for each time point and gene set. e–h, Signal across all cohorts with longitudinal data: gestational age model (e), placenta (f), developing heart (g) and collagen ECM (h). Linear fits are shown of transcriptome fractions for all samples across corresponding gestational ages recorded at collection times. The band around the solid line corresponds to the 95% confidence interval. All slopes for the gestational age coefficients are distinct from 0 at a confidence level of 0.05. Cohort is indicated by colour.