Fig. 2. SWI/SNF ATPase degradation disrupts physical chromatin accessibility at the core-enhancer circuitry to disable oncogenic transcriptional programs.
a, ATAC-seq read-density heat maps from VCaP cells treated with DMSO or AU-15330 for indicated durations (n = 2 biological replicates). b, Genome-wide changes in chromatin accessibility upon AU-15330 treatment for 4 h in VCaP cells along with genomic annotation of sites that lose physical accessibility (lost) or remain unaltered (retained). c, d, ChIP–seq read-density heat maps for AR and FOXA1 (c) and H3K27Ac (d) at the AU-15330 (AU)-compacted genomic sites in VCaP cells after treatment with DMSO or AU-15330 (1 μM) for indicated times and stimulation with R1881 (1 nM, 3 h). e, RNA-seq heat maps for classical AR target genes in LNCaP, VCaP and LAPC4 prostate cancer cells with or without 24 h of AU-15330 treatment.