Extended Data Fig. 7. CVnCoV and CV2CoV protect the lungs from pathological changes upon viral challenge (6/group).
Eight lung lobes (4 sections from right and left, caudal to cranial) were assessed and scored (1-4) for each of the following lesions: 1) Interstitial inflammation and septal thickening 2) Eosinophilic interstitial infiltrate 3) Neutrophilic interstitial infiltrate 4) Hyaline membranes 5) Interstitial fibrosis 6) Alveolar infiltrate, macrophage 7) Alveolar/Bronchoalveolar infiltrate, neutrophils 8) Syncytial cells 9) Type II pneumocyte hyperplasia 10) Broncholar infiltrate, macrophage 11) Broncholar infiltrate, neutrophils 12) BALT hyperplasia 13) Bronchiolar/peribronchiolar inflammation 14) Perivascular, mononuclear infiltrates 15) Vessels, endothelialitis. Each feature assessed was assigned a score of 0 = no significant findings; 1 = minimal; 2 = mild; 3 = moderate; 4 = marked/severe. (a) Cumulative scores per animal (b) Cumulative scores per lung lobe. Individual animals are represented by symbols. Representative histopathology from sham vaccinated (c-h), CnVCoV vaccinated (i, j), and Cv2CoV vaccinated (k, l) animals showing (c, d, inset) alveolar macrophage infiltrate, (e, f, inset) syncytial cells (arrowheads) and type II pneumocyte hyperplasia, inset (g, h, inset) bronchiolar epithelial necrosis with neutrophilic infiltrates (i) alveolar neutrophilic infiltrate and alveolar septal thickening (j) focal consolidation with inflammation composed of macrophages, neutrophils, and syncytial cells (k) focal pneumocyte hyperplasia, syncytial cells and inflammatory infiltrates (l) peribronchiolar inflammation. Statistical analysis was performed using two-tailed nonparametric Mann-Whitney test. Scale bars: 100 microns (c), 50 microns (e, g) 20 microns (i-l). BALT bronchus associated lymphoid tissue.