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. 2021 Mar 17;91(1):73–82. doi: 10.1038/s41390-021-01457-y

Fig. 3. Necrostatin-1s inhibit the expression of necroptotic proteins.

Fig. 3

a Western blotting of necroptosis-related proteins. b Western blotting of phosphorylated caspase-8 (cleaved bands). c Relative protein density of the above-mentioned proteins. n = 6 in each group. d Double immunofluorescent staining of CD4 and pRIPK3 in each group. Magnification = ×200, bar = 50 μm. pRIPK1 phosphorylated RIPK1, pRIPK3 phosphorylated RIPK3, pMLKL phosphorylated MLKL, BF breastfeeding under a physiological status, NEC neonatal mice that underwent formula feeding and cold/asphyxia stress, DMSO 10% DMSO in PBS, the dissolvent of necrostatin-1s, nec-1s necrostatin-1s. NEC+DMSO vs. NEC+Nec-1s, **p < 0.01 and ***p < 0.001.