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. 2021 Mar 17;91(1):73–82. doi: 10.1038/s41390-021-01457-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a The effect of TRIF siRNA transfection, as confirmed by Western blot analysis. b Western blotting and c relative protein density of necroptotic proteins after LPS challenge in the scramble and the TRIF silenced HCT-116 cells. d The effect of TNFR1 siRNA transfection, as confirmed by Western blot analysis. e Western blotting and f relative protein density of necroptotic proteins after LPS challenge in the scramble and the TNFR1-silenced HCT-116 cells. g Simultaneous TRIF and TNFR1 siRNA transfections resulted in near-complete depletion of both TRIF and TNFR1 expressions, as confirmed by Western blot analysis. h Western blotting and i relative protein density of necroptotic proteins after LPS challenge in the scramble and the TRIF/TNFR1-silenced HCT-116 cells. pRIPK1 phosphorylated RIPK1, pRIPK3 phosphorylated RIPK3, pMLKL phosphorylated MLKL. ^#p > 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001. n = 4 in each group.