Table 2. Cubic spline model used to determine the LD₅₀, LD₉₀ and LD₁₀₀ of E. chlorantha bark ethanolic extract (HAE), C. citratus leaves HAE, A. indica seed HAE and G. lucida bark HAE.

Plant material	Spline intervals	t^3	t^2	t	Constant	R 2
Enantia chlorantha bark HAE	(0; 5)	−0.005	0.000	0.119	100.000	0.92
	(5; 12.5)	0.002	−0.072	−0.239	100.000
	(12.5; 25)	0.001	−0.028	−0.986	95.000
	(25; 50)	0.000	0.005	−1.278	80.000
	(50; 100)	0.000	0.000	−1.163	50.000
	(100; 200)	0.000	0.010	−0.668	0.000
Cymbopogon citratus leaves HAE	(0; 5)	0.003	0.000	−0.067	100.000	0.88
	(5; 12.5)	−0.008	0.040	0.133	100.000
	(12.5; 25)	0.004	−0.133	−0.567	100.000
	(25; 50)	0.000	0.019	−2.000	80.000
	(50; 100)	0.000	0.011	−1.264	40.000
	(100; 200)	0.000	0.006	−0.413	0.000
Azadirachta indica seed HAE	(0; 5)	0.038	0.000	−9.951	100.000	0.69
	(5; 12.5)	−0.021	0.570	−7.099	55.000
	(12.5; 25)	−0.002	0.099	−2.080	25.000
	(25; 50)	0.000	0.013	−0.676	10.000
	(50; 100)	0.000	0.006	−0.186	0.000
	(100; 200)	0.000	−0.001	0.068	0.000
Garcinia lucida bark HAE	(0; 5)	−0.017	0.000	−0.584	100.000	0.85
	(5; 12.5)	0.018	−0.249	−1.831	95.000
	(12.5; 25)	−0.005	0.165	−2.468	75.000
	(25; 50)	0.001	−0.025	−0.721	60.000
	(50; 100)	0.000	0.016	−0.937	35.000
	(100; 200)	0.000	0.000	−0.137	15.000