TABLE 1.
Characteristics of M. bohemicum isolates from various sources compared with type strains of M. bohemicum and M. interjectum
| Clustera and strain or isolate(s) by source (n) | Fatty markers
|
Mycolic acids
|
Pigment | Growth at 42°Cb | Nitrate reductionb | Semiquantitative catalase (45 mm foam)b | Tween hydrolysisb | Arylsulfatase (10 d)b | Ureaseb | Pyrazinamidaseb | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 18:0alc | 20:0alc | 2-Me-20:0 | 2,4-diMe- 22:0 | 2,4,6-triMe- 22:0 | 2,4,6-triMe- 24:0 | 26:0 | TLC | HPLC | |||||||||
| A. M. bohemicum DSM 44277Tc | 5.3 | 1.8 | 1.7 | 0.6 | 0.9 | 1.7 | 0 | NDd | Typical | Yellow | + | − | + | − | − | + | + |
| A. Clinical isolates (8)e | 5.1 ± 0.8f | 1.4 ± 0.4 | 3.0 ± 1.2 | 1.1 ± 0.5 | 1.6 ± 1.0 | 2.2 ± 0.9 | 0 | α, metoxy, keto, dicarboxy | Typicalg | Yellow | + | v | + | − | v | + | − |
| A. Veterinary isolate (1) | 5.6 | 1.4 | 3.7 | 1.3 | 2.1 | 2.1 | 0 | ND | Typical | Yellow | + | − | + | − | +w | + | − |
| Environmental isolates | |||||||||||||||||
| A. E170-2, E445 | 5.5–6.0h | 0.7–1.4 | 2.9–3.6 | 0.8–1.0 | 1.1–1.2 | 2.0–2.7 | 0 | ND | Typical | Yellow | + | v | + | − | + | + | − |
| B. E743, E744 | 4.5–4.9h | 0 | 1.9–3.1 | 0.2–0.6 | 0.5–1.9 | 1.0–1.5 | 0 | α, metoxy, keto, dicarboxy | Typical | Yellow | + | v | + | + | + | v | + |
| C. E590 | 0 | 0 | 2.5 | 0.5 | 7.5 | 1.6 | 0 | ND | Atypical | Yellow | + | + | + | + | + | + | + |
| M. interjectum ATCC 51457Tc | 5.5 | 1.8 | 4.1 | 2.3 | 0.0 | 2.2 | 5.1 | α, keto, dicarboxy | ND | Yellow | − | + | + | + | − | + | + |
a
Clustering (A, B, and C) was based on ITS sequences, GLC fatty acids, and HPLC.
b
Grading of results: +, positive; −, negative; +w, weak positive; v, variable.
c
Results of the present study.
d
ND, not done.
e
Seven isolates from Finland and one from Germany; see Materials and Methods.
f
Percent of total peak area (mean ± SD).
g
Five of eight isolates analyzed; see Materials and Methods.
h
Percent of total peak area (range).